"This book is dedicated to Metabolic Flux Analysis in systems that can be studied under metabolic steady-state or pseudo-steady-state conditions, also called stationary approaches. Under these conditions the change of intracellular metabolite concentrations ( CMET ) as a function of the stoichiometric matrix S and the flux vector v can be approximated to zero. This approximation converts the equations system from a differential to a linear one and greatly simplifies its solution. We hope that this book will open the field of metabolic flux analysis to a wider scientific community by providing helpful tricks to those who want to start a new flux analysis project but are overwhelmed by the complexity of the approach. We tried to break it up into manageable bits of information that can be implemented step by step following the methods chapters provided. The overview chapters are there to explain the necessary basics to get started and also to review what has been achieved within a few model organisms."--