Flow cytometry is an increasingly widely used technique for measuring the properties of single cells and for separating cells and sub-cellular particles. An understanding of this technology is becoming essential for many non-specialists. Flow Cytometry is a practical guide to the instrumentation and the application of this method in mammalian cell biology. Information is included on fluorescence and immunofluorescence, DNA analysis, chromosome analysis and sorting, study of cell proliferation and death, and choice of instrument. Flow Cytometry is essential reading for all workers wishing to use this technique, whether cell biologists, immunologists or pathologists.