This study presents the famous city of Babylon in its latest phase of occupation: from the end of the Achaemenid period (second half of the fourth century B.C.), during the reign of Alexander, the Successors, the Seleucid and Arsacid dynasty until the very end of cuneiform literature and other historical sources (around third-fourth century AD). It contains first of all a survey of the available Classical and Oriental sources (chapter 1), a topography of the city (chapter 2), an overview of political events and Babylon's role in the Empire (chapter 3). Furthermore Babylon's institutions (chapter 4), its social and economic (chapter 5), religious (chapter 6) and cultural (chapter 7) life are discussed. Finally, Babylon's legacy and its significance for later cultures appears in chapter 8.