The 81st Cold Spring Harbor Symposium focused on Targeting Cancer. The implications of how the underlying science can drive improvements in diagnostic, prognostic, and therapeutic approaches was a major theme throughout the Symposium. The topics covered at the meeting included: Cancer Genes and Genomes; Cancer Pathways; Tumor Cell Biology; Cancer Growth & Progression; Innate & Adaptive Immune Responses; and Enabling Technologies. Several speakers participated in interviews during the course of the Symposium week and transcripts of those videos and the Dorcas Cummings lecture are included.