Flow cytometry is a technique of increasing importance in a wide range of biological applications. Authoritative and detailed guidance is essential if the user is to optimize the data obtained. This second edition of Flow Cytometry provides a complete update on the original volume. It covers fluorescence technology, DNA analysis and the measurement of cell proliferation, chromosome analysis and sorting, membrane potential and pH, as well as many other applications to cell biology. New authors have written enlarged chapters on the important topics of immunofluorescence of surface markers and the measurement of cytoplasmic and nuclear antigens. Other sections of the coverage have also been expanded substantially where necessary. . Flow Cytometry: A Practical Approach will be of great value to all researchers in biological and medical laboratories who currently use, or wish to use, a flow cytometer in their work. It provides clear practical guidance for those new to the technology and a timely update for more experienced personnel.