This book constitutes the refereed proceedings of the 16th International Conference on Text, Speech and Dialogue, TSD 2013, held in Pilsen, Czech Republic, in September 2013. The 65 papers presented together with 5 invited talks were carefully reviewed and selected from 148 submissions. The main topics of this year's conference was corpora, texts and transcription, speech analysis, recognition and synthesis, and their intertwining within NL dialogue systems. The topics also included speech recognition, corpora and language resources, speech and spoken language generation, tagging, classification and parsing of text and speech, semantic processing of text and speech, integrating applications of text and speech processing, as well as automatic dialogue systems, and multimodal techniques and modelling.